Bif-1 Recombinant monoclonal antibody

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  WB analysis using 83715-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83715-1-RR (Bif-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 83715-1-RR

  WB result of Bif-1 antibody (83715-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Bif-1 transfected HepG2 cells.

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  IF Staining of HeLa using 83715-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Bif-1 antibody (83715-1-RR, Clone: 240623E1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83715-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83715-1-RR against Human Bif-1 were performed. The affinity constant is 0.71 nM.

• Featured Product
• KD/KO Validated

Bif-1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83715-1-RR

Clone No.240623E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

SH3GLB1, 240623E1, Bax interacting factor 1, Bax-interacting factor 1, Bif1

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Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells, K-562 cells, HEK-293 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83715-1-RR targets Bif-1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7710

  Product name: Recombinant human SH3GLB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-365 aa of BC007455

  Sequence: MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIMKQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIKCGETQKRIGTADRELIQTSALNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLKKAKAAETRNSSEQELRITQSEFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQCYQYMLDLQKQLGSFPSNYLSNNNQTSVTPVPSVLPNAIGSSAMASTSGLVITSPSNLSDLKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERGNQKGKVPITYLELLN

• Full Name: SH3-domain GRB2-like endophilin B1
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC007455
• Gene Symbol: Bif-1
• Gene ID (NCBI): 51100
• RRID: AB_3671317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y371
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SH3GLB1 also known as Bif-1, contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain, but shares no significant homology with members of the Bcl-2 family. SH3GLB1 is involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588).