Anti-Biotin Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of human PBMCs using 98598-1-RR (same clone as 98598-1-PBS)

  1x10^6 human PBMCs were surface stained with Biotin Anti-Human CD13 (WM15) (Biotin-65186, Clone: WM15), and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone: 242355B1) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98598-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98598-1-RR (same clone as 98598-1-PBS)

  1x10^6 human PBMCs were surface stained with Biotin Anti-Human CD13 (WM15) (Biotin-65186, Clone: WM15) and then stained with 0.25 ug Anti-Biotin Rabbit RecAb (98598-1-RR, Clone:242355B1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98598-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98598-1-RR (same clone as 98598-1-PBS)

  1x10^6 mouse splenocytes were surface stained with FcZero-rAb™ Biotin Anti-Mouse CD4 (RM4-4) Rabbit IgG Recombinant Antibody (Biotin-FcA65655, Clone: RM4-4), and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone:242355B1) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98598-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98598-1-RR (same clone as 98598-1-PBS)

  1x10^6 mouse splenocytes were surface stained with FcZero-rAb™ Biotin Anti-Mouse CD4 (RM4-4) Rabbit IgG Recombinant Antibody (Biotin-FcA65655, Clone: RM4-4) and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone: 242355B1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98598-1-PBS in a different storage buffer formulation.

Biotin Uni-rAb^® Recombinant Antibody for FC

Cat No. 98598-1-PBS

Clone No.242355B1

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

FC

Synonyms

Vitamin B7, Vitamin H

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Product Information

98598-1-PBS targets Biotin in FC applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Biotin-KLH

• Full Name: Biotin
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Biotin, also called vitamin H or vitamin B7, is a water-soluble vitamin required by all organisms (PMID: 12055344). It serves as a necessary cofactor for enzymes involved in carboxylation reactions essential for gluconeogenesis, lipid metabolism, and amino acid catabolism (PMID: 38825069). Biotin is often used to label proteins and nucleic acids. This anti-biotin antibody can be used for detecting biotinylated molecules.