1x10^6 human PBMCs were surface stained with Biotin Anti-Human CD13 (WM15) (Biotin-65186, Clone: WM15), and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone: 242355B1) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human PBMCs were surface stained with Biotin Anti-Human CD13 (WM15) (Biotin-65186, Clone: WM15), and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone: 242355B1) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human PBMCs using 98598-1-RR
1x10^6 human PBMCs were surface stained with Biotin Anti-Human CD13 (WM15) (Biotin-65186, Clone: WM15) and then stained with 0.25 ug Anti-Biotin Rabbit RecAb (98598-1-RR, Clone:242355B1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human PBMCs were surface stained with Biotin Anti-Human CD13 (WM15) (Biotin-65186, Clone: WM15) and then stained with 0.25 ug Anti-Biotin Rabbit RecAb (98598-1-RR, Clone:242355B1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of mouse splenocytes using 98598-1-RR
1x10^6 mouse splenocytes were surface stained with FcZero-rAb™ Biotin Anti-Mouse CD4 (RM4-4) Rabbit IgG Recombinant Antibody (Biotin-FcA65655, Clone: RM4-4), and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone:242355B1) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 mouse splenocytes were surface stained with FcZero-rAb™ Biotin Anti-Mouse CD4 (RM4-4) Rabbit IgG Recombinant Antibody (Biotin-FcA65655, Clone: RM4-4), and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone:242355B1) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of mouse splenocytes using 98598-1-RR
1x10^6 mouse splenocytes were surface stained with FcZero-rAb™ Biotin Anti-Mouse CD4 (RM4-4) Rabbit IgG Recombinant Antibody (Biotin-FcA65655, Clone: RM4-4) and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone: 242355B1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 mouse splenocytes were surface stained with FcZero-rAb™ Biotin Anti-Mouse CD4 (RM4-4) Rabbit IgG Recombinant Antibody (Biotin-FcA65655, Clone: RM4-4) and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone: 242355B1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
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FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98598-1-RR targets Biotin in FC applications and shows reactivity with n/a samples.
Store at 2 - 8°C. Stable for one year after shipment.
Background Information
Biotin, also called vitamin H or vitamin B7, is a water-soluble vitamin required by all organisms (PMID: 12055344). It serves as a necessary cofactor for enzymes involved in carboxylation reactions essential for gluconeogenesis, lipid metabolism, and amino acid catabolism (PMID: 38825069). Biotin is often used to label proteins and nucleic acids. This anti-biotin antibody can be used for detecting biotinylated molecules.
1x10^6 human PBMCs were surface stained with Biotin Anti-Human CD13 (WM15) (Biotin-65186, Clone: WM15), and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone: 242355B1) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of human PBMCs using 98598-1-RR
1x10^6 human PBMCs were surface stained with Biotin Anti-Human CD13 (WM15) (Biotin-65186, Clone: WM15) and then stained with 0.25 ug Anti-Biotin Rabbit RecAb (98598-1-RR, Clone:242355B1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of mouse splenocytes using 98598-1-RR
1x10^6 mouse splenocytes were surface stained with FcZero-rAb™ Biotin Anti-Mouse CD4 (RM4-4) Rabbit IgG Recombinant Antibody (Biotin-FcA65655, Clone: RM4-4), and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone:242355B1) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
FC experiment of mouse splenocytes using 98598-1-RR
1x10^6 mouse splenocytes were surface stained with FcZero-rAb™ Biotin Anti-Mouse CD4 (RM4-4) Rabbit IgG Recombinant Antibody (Biotin-FcA65655, Clone: RM4-4) and then stained with 0.25 ug Anti--Biotin Rabbit RecAb (98598-1-RR, Clone: 242355B1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Biotin Recombinant antibody
Catalog Number
98598-1-RR
Citations
-
Dilutions
Applications
FC
Reactivity
n/a
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
