PE Anti-Biotin Rabbit Recombinant Antibody

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  IF Staining of HepG2 using PE-98598

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Biotin-conjugated PEX14 Polyclonal antibody (Biotin-10594) at dilution of 1:200, and PE Anti-Biotin Rabbit Recombinant Antibody (PE-98598, Clone: 242355B1) at dilution of 1:200.

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  FC experiment of human PBMCs using PE-98598

  1x10^6 human PBMCs were surface stained with Biotin Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (Biotin-65569, Clone: OKT3), and then stained with 0.1 ug PE Anti-Biotin Rabbit RecAb (PE-98598, Clone: 242355B1) or 0.1 ug PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using PE-98598

  1x10^6 human PBMCs were surface stained with Biotin Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (Biotin-65569, Clone: OKT3), and then stained with 0.1 ug PE Anti-Biotin Rabbit RecAb (PE-98598, Clone:242355B1) (red) or 0.1 ug PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of mouse splenocytes using PE-98598

  1x10^6 mouse splenocytes were surface stained with Biotin Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rat IgG2a Recombinant Antibody (Biotin-65663, Clone: M5/114.15.2), and then stained with 0.1 ug PE Anti-Biotin Rabbit RecAb (PE-98598, Clone: 242355B1) or 0.1 ug PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PE-98598

  1x10^6 mouse splenocytes were surface stained with Biotin Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rat IgG2a Recombinant Antibody (Biotin-65663, Clone: M5/114.15.2), and then stained with 0.1 ug PE Anti-Biotin Rabbit RecAb (PE-98598, Clone: 242355B1) (red) or 0.1 ug PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Biotin Uni-rAb^® Recombinant Antibody for IF/ICC, FC

Cat No. PE-98598

Clone No.242355B1

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

IF/ICC, FC

Synonyms

Vitamin B7, Vitamin H

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC detected in: human PBMCs, mouse splenocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.1 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-98598 targets Biotin in IF/ICC, FC applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Biotin-KLH

• Full Name: Biotin
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Excitation Laser: Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Biotin, also called vitamin H or vitamin B7, is a water-soluble vitamin required by all organisms (PMID: 12055344). It serves as a necessary cofactor for enzymes involved in carboxylation reactions essential for gluconeogenesis, lipid metabolism, and amino acid catabolism (PMID: 38825069). Biotin is often used to label proteins and nucleic acids. This anti-biotin antibody can be used for detecting biotinylated molecules.