Brevican Polyclonal antibody, PBS Only

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• Brevican Polyclonal antibody, PBS Only (19017-1-PBS)

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  WB analysis of human brain using 19017-1-AP (same clone as 19017-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 19017-1-AP (same clone as 19017-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 19017-1-AP (same clone as 19017-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 19017-1-AP (same clone as 19017-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 19017-1-AP (Brevican antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 19017-1-AP (same clone as 19017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 19017-1-AP (same clone as 19017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19017-1-AP (Brevican antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 19017-1-AP (same clone as 19017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19017-1-AP (Brevican antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19017-1-PBS in a different storage buffer formulation.

Brevican Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 19017-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

19017-1-PBS targets Brevican in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13552

  Product name: Recombinant human BCAN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 23-350 aa of BC010571

  Sequence: LEGDSSEDRAFRVRIAGDAPLQGVLGGALTIPCHVHYLRPPPSRRAVLGSPRVKWTFLSRGREAEVLVARGVRVKVNEAYRFRVALPTYPASLTDVSLALNELRPNDSGIYRCEVQHGIDDSSDAVEVKVKGVVFLYREGSARYAFSFSGAQEACARIGAHIATPEQLYAAYLGGYEQCDAGWLSDQTVRYPIQTPREACYGDMDGFPGVRNYGVVDPDDLYDVYCYAEDLNGELFLGDPPEKLTLEEARAYCQERGAEIATTGQLYAAWDGGLDHCSPGWLADGSVRYPIVTPSQRCGGGLPGVKTLFLFPNQTGFPNKHSRFNVY

• Full Name: brevican
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 145 kDa, 72kDa, 80 kDa
• GenBank Accession Number: BC010571
• Gene Symbol: BCAN
• Gene ID (NCBI): 63827
• RRID: AB_10643526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96GW7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Brevican is a brain-specific proteoglycan belonging to the aggrecan family that share a number of structural features. Brevican contains a hyaluronic acid-binding domain in its N-terminus and a lectin-like domain in its C-terminus. Expression of brevican is highly specific in the brain and increases as the brain develops. It may play a role in maintaining the extracellular environment of mature brain as a major constituent of the adult brain extracellular matrix (PMID:9117262). Brevican core protein exists as a 145 kDa full-length form and a 80 kDa N terminally truncated form (PMID: 8144512). This antibody can recognize the 72 kDa isoform of target.