EDRF1 Polyclonal antibody

EDRF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21883-1-AP

Synonyms

C10orf137, RP11 383C5.6, Erythroid differentiation-related factor 1

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Validation Data Gallery

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  WB analysis using 21883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21883-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 21883-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21883-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21883-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21883-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21883-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 21883-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21883-1-AP (EDRF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 21883-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21883-1-AP (EDRF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 21883-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21883-1-AP (EDRF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 21883-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 21883-1-AP (EDRF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 21883-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 21883-1-AP (EDRF1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, PC-3 cells, HEK-293 cells, mouse brain tissue, MCF-7 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

21883-1-AP targets EDRF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EDRF1 fusion protein Ag16441
• Full Name: chromosome 10 open reading frame 137
• Calculated Molecular Weight: 1238 aa, 139 kDa
• Observed Molecular Weight: 140-160 kDa
• GenBank Accession Number: BC107479
• Gene Symbol: EDRF1
• Gene ID (NCBI): 26098
• RRID: AB_11042452
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EDRF1, also named as C10orf137, is a transcription factor involved in erythroid differentiation. It regulates alpha- and gamma-globin gene synthesis by modulating DNA-binding activity of GATA-1 transcription factor. EDRF1 has four isoforms with MW 66 kDa, 89 kDa and 135-140 kDa.

Publications

Species	Application	Title
human	WB	iScience; Differential localization of dengue virus protease affects cell homeostasis and triggers to thrombocytopenia; Authors - Lekha Gandhi