C10orf58 Polyclonal antibody

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• C10orf58 Polyclonal antibody (20446-1-AP)

C10orf58 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20446-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

F213A, FAM213A, PAMM, Peroxiredoxin-like 2 activated in M-CSF stimulated monocytes, Peroxiredoxin-like 2A

• 

  WB analysis of ROS1728 using 20446-1-AP

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 20446-1-AP (C10orf58 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 20446-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20446-1-AP (C10orf58 antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 20446-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20446-1-AP (C10orf58 antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of ROS1728 using 20446-1-AP

  Immunofluorescent analysis of ROS1728 cells using 20446-1-AP (C10orf58 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of ROS1728 using 20446-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed ROS1728 cells using C10orf58 antibody (20446-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: ROS1728 cells
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: ROS1728 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20446-1-AP targets C10orf58 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14272

  Product name: Recombinant human C10orf58 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 35-229 aa of BC005871

  Sequence: DVFLSKPQKAALEYLEDIDLKTLEKEPRTFKAKELWEKNGAVIMAVRRPGCFLCREEAADLSSLKSMLDQLGVPLYAVVKEHIRTEVKDFQPYFKGEIFLDEKKKFYGPQRRKMMFMGFIRLGVWYNFFRAWNGGFSGNLEGEGFILGGVFVVGSGKQGILLEHREKEFGDKVNLLSVLEAAKMIKPQTLASEKK

• Full Name: chromosome 10 open reading frame 58
• Calculated Molecular Weight: 229 aa, 26 kDa
• Observed Molecular Weight: 24-30 kDa
• GenBank Accession Number: BC005871
• Gene Symbol: C10orf58
• Gene ID (NCBI): 84293
• RRID: AB_2878687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BRX8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C10orf58, also name as FAM213A and PAMM, is involved in redox regulation of the cell. It acts as an antioxidant. C10orf58 inhibits TNFSF11-induced NFKB1 and JUN activation and osteoclast differentiation. It may affect bone resorption and help to maintain bone mass. The MW of C10orf58 is 24-30 kDa.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Rep; The miR4261/PAMM axis in multiple myeloma promotes bone resorption.; Authors - Jiangxia Cui ; KD Validated
Human	IHC	Oncol Rep; Overexpression of ZNF169 promotes the growth and proliferation of colorectal cancer cells via the upregulation of ANKZF1; Authors - Jie Zhang