SDH5 Polyclonal antibody

SDH5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 19906-1-AP

Synonyms

C11orf79, hSDH5, PGL2, SDH assembly factor 2, SDH5, SDHAF2, SDHAF2, PGL2, SDH5

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Validation Data Gallery

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  WB analysis using 19906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19906-1-AP (SDH5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19906-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 19906-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 19906-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 19906-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 19906-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SDH5 antibody (19906-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human testis tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

19906-1-AP targets SDH5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SDH5 fusion protein Ag13893
• Full Name: chromosome 11 open reading frame 79
• Calculated Molecular Weight: 166 aa, 20 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC002331
• Gene Symbol: SDH5
• Gene ID (NCBI): 54949
• RRID: AB_2878621
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SDH5, also named asC11orf79, SDHAF2 and SDHAF2, is a mitochondrial protein needed for the flavination of a succinate dehydrogenase complex subunit required for activity of the complex. SDH5 has been shown to be mutated in patients with paragangliomas, also known as familial non-chromaffin paragangliomas type 2. Catalog#19906-1-AP is a rabbit polyclonal antibody raised against the full-length of human SDH5.

Publications

Species	Application	Title
human	WB	Free Radic Biol Med; SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.; Authors - Yuanzhen Ma
human	WB	Apoptosis; GAS6/Axl is associated with AMPK activation and attenuates H2O2-induced oxidative stress; Authors - Zhenxing Liang
mouse	WB	Cell Biochem Funct; Pterostilbene protects against H2 O2 -induced oxidative stress by regulating GAS6/Axl signaling in HL-1 cells; Authors - Wencheng Di