C11orf80 Polyclonal antibody

C11orf80 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25957-1-AP

Synonyms

C11orf80

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Validation Data Gallery

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  WB analysis of L02 using 25957-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 25957-1-AP (C11orf80 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 25957-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25957-1-AP (C11orf80 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 25957-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25957-1-AP (C11orf80 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 25957-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25957-1-AP (C11orf80 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 25957-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25957-1-AP (C11orf80 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: L02 cells
• Positive IHC detected in: human pancreas tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25957-1-AP is C11orf80 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C11orf80 fusion protein Ag22895
• Full Name: chromosome 11 open reading frame 80
• Calculated Molecular Weight: 522 aa, 58 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC028240
• Gene Symbol: C11orf80
• Gene ID (NCBI): 79703
• RRID: AB_2880309
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C11orf80 locates at the chromosome 11. It has two isoforms with MW 70-75 kDa.