Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, Jurkat cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human cervical cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19892-1-AP targets ASUN in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13727 Product name: Recombinant human C12orf11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 357-706 aa of BC003081 Sequence: GRSVLLEQPRKSGSKVISHMLSSHGGEIFLHVLSSSRSILEDPPSISEGCGGRVTDYRITDFGEFMRENRLTPFLDPRYKIDGSLEVPLERAKDQLEKHTRYWPMIISQTTIFNMQAVVPLASVIVKESLTEEDVLNCQKTIYNLVDMERKNDPLPISTVGTRGKGPKRDEQYRIMWNELETLVRAHINNSEKHQRVLECLMACRSKPPEEEERKKRGRKREDKEDKSEKAVKDYEQEKSWQDSERLKGILERGKEELAEAEIIKDSPDSPEPPNKKPLVEMDETPQVEKSKGPVSLLSLWSNRINTANSRKHQEFAGRLNSVNNRAELYQHLKEENGMETTKNGKASRQ Predict reactive species |
| Full Name | chromosome 12 open reading frame 11 |
| Calculated Molecular Weight | 706 aa, 80 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC003081 |
| Gene Symbol | ASUN |
| Gene ID (NCBI) | 55726 |
| RRID | AB_10638473 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NVM9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Asunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization during Drosophila spermatogenesis (PMID: 19357193). Mammalian and Drosophila ASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ASUN antibody 19892-1-AP | Download protocol |
| IHC protocol for ASUN antibody 19892-1-AP | Download protocol |
| IP protocol for ASUN antibody 19892-1-AP | Download protocol |
| WB protocol for ASUN antibody 19892-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ASUN polyclonal antibody (Cat# 19892-1-AP) has 5 total citations. These appear in Nature Communications (2), Molecular Cell, FASEB Journal, and International Journal of Clinical and Experimental Pathology. The research fields span RNA polymerase II pause/release regulation by the Integrator complex, developmental ciliopathy, stress granule disassembly linked to ALS pathogenesis, ZBTB26-mediated transcriptional activation, and FOXM1-driven breast cancer cell migration.
| Species | Application | Title |
|---|---|---|
Nat Commun Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes. | ||
Nat Commun INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex | ||
Mol Cell Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis. | ||
Int J Clin Exp Pathol Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration. |
