ASUN Polyclonal antibody

ASUN Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19892-1-AP

Synonyms

ASUN, C12orf11, GCT1, NET48, NY SAR 95, Sarcoma antigen NY SAR 95

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Validation Data Gallery

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  WB analysis using 19892-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19892-1-AP (ASUN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 19892-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19892-1-AP (ASUN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 19892-1-AP

  IP result of anti-ASUN (IP:19892-1-AP, 4ug; Detection:19892-1-AP 1:4000) with MCF-7 cells lysate 1040ug.

• 

  IHC staining of human cervical cancer using 19892-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19892-1-AP (ASUN antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 19892-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19892-1-AP (ASUN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 19892-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19892-1-AP (ASUN antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 19892-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19892-1-AP (ASUN antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human cervical cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

19892-1-AP targets ASUN in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASUN fusion protein Ag13727
• Full Name: chromosome 12 open reading frame 11
• Calculated Molecular Weight: 706 aa, 80 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC003081
• Gene Symbol: C12orf11
• Gene ID (NCBI): 55726
• RRID: AB_10638473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization during Drosophila spermatogenesis (PMID: 19357193). Mammalian and Drosophila ASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494).

Publications

Species	Application	Title
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Nat Commun; INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex; Authors - Lauren G. Mascibroda
human	ChIP, IP, WB	Nat Commun; Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes.; Authors - Bernd Stadelmayer
human	WB	Int J Clin Exp Pathol; Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.; Authors - Xiaojuan Ye

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