RESF1 Polyclonal antibody

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• RESF1 Polyclonal antibody (33680-1-AP)

• 

  WB analysis using 33680-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33680-1-AP (C12orf35 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 33680-1-AP

  WB result of C12orf35 antibody (33680-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C12orf35 transfected K-562 cells.

• Featured Product
• KD/KO Validated

RESF1 Polyclonal Antibody for WB, ELISA

Cat No. 33680-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C12orf35, KIAA1551, Retroelement silencing factor 1

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Tested Applications

• Positive WB detected in: Caco-2 cells, K-562 cells, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33680-1-AP targets RESF1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38168

  Product name: Recombinant human C12orf35 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1399-1747 aa of BC140819

  Sequence: GSVGATFKLGDSLSNPNERAIVKEKMVSNTKSVDTKASSSKFSRILTPKEYLQRQKHKEALSNKASKKICVKNVPCDSEHMRPSKLAVQVESCGKSNEKHSSGVQTSKESLNGLTSHGKNLKIHHSQESKTYNILRNVKEKVGGKQPDKIWIDKTKLDKLTNISNEAQFSQMPPQVKDQKKLYLNRVGFKCTERESISLTKLESSPRKLHKDKRQENKHKTFLPVKGNTEKSNMLEFKLCPDILLKNTNSVEERKDVKPHPRKEQAPLQVSGIKSTKEDWLKFVATKKRTQKDSQERDNVNSRLSKRSFSADGFEMLQNPVKDSKEMFQTYKQMYLEKRSRSLGSSPVK

• Full Name: chromosome 12 open reading frame 35
• Calculated Molecular Weight: 1747 aa, 195 kDa
• Observed Molecular Weight: 240-250 kDa
• GenBank Accession Number: BC140819
• Gene Symbol: C12orf35
• Gene ID (NCBI): 55196
• RRID: AB_3743047
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9HCM1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RESF1 (Retroelement Silencing Factor 1, also known as C12orf35 or KIAA1551) is a nuclear protein that primarily mediates the epigenetic silencing of endogenous retroviruses (ERVs) and imprinted genes by interacting with the SETDB1 histone methyltransferase, thereby maintaining H3K9me3 modifications and heterochromatin conformation. Recent studies have revealed that RESF1 exerts metastasis-suppressive functions in estrogen receptor-negative breast cancer. Additionally, RESF1 negatively regulates ribosomal biogenesis and global protein translation, serving as a multifunctional factor that links genome stability, stem cell pluripotency, and tumor suppression.