C13orf3 Polyclonal antibody

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  WB analysis using 26320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26320-1-AP (C13orf3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

C13orf3 Polyclonal Antibody for WB, ELISA

Cat No. 26320-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

RAMA1, SKA3, Spindle And Kinetochore Associated Complex Subunit 3, Spindle and kinetochore-associated protein 3

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Tested Applications

• Positive WB detected in: HEK-293T cells, HL-60 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26320-1-AP targets C13orf3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C13orf3 fusion protein Ag23684
• Full Name: chromosome 13 open reading frame 3
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC048988
• Gene Symbol: C13orf3
• Gene ID (NCBI): 221150
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IX90
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C13orf3 (now known as Ska3) is a component of a spindle- and mitophore-associated protein complex that regulates microtubule attachment to the mitophore during mitosis. Ska3 is localized to the outer side of the mitophore during mitosis and may be essential for normal chromosome segregation. In addition, deletion of Ska3 results in a delay in mid-mitotic division and an inability to silence the spindle checkpoint or enter prophase. Accumulation of Ska3 is dependent on Sgo1 proteins, whereas mitotic accumulation of Sgo1 in prophase is not dependent on Ska3. The discovery of Ska3 further refines the function of the Ska complex, which plays an important role in mitotic progression and chromosome segregation. The discovery of Ska3 in the prophase of Sgo1 is a key factor in mitosis and chromosome segregation.