EMC7/C15orf24 Polyclonal antibody

EMC7/C15orf24 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 27550-1-AP

Synonyms

C11orf3, C15orf24, EMC7, ER membrane protein complex subunit 7, HT022, ORF1 FL1, UNQ905/PRO1926, UPF0480 protein C15orf24

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Validation Data Gallery

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  WB analysis using 27550-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27550-1-AP (C15orf24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

27550-1-AP targets EMC7/C15orf24 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EMC7/C15orf24 fusion protein Ag26784
• Full Name: chromosome 15 open reading frame 24
• Observed Molecular Weight: 20-26 kDa
• GenBank Accession Number: BC104934
• Gene Symbol: C15orf24
• Gene ID (NCBI): 56851
• RRID: AB_2880902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian ER membrane complex (EMC) is composed of 10 subunits, EMC 1-10. Endoplasmic reticulum membrane protein complex subunit 7(EMC7, also named C15orf24) is part of the endoplasmic reticulum membrane protein complex (EMC) that enables theenergy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins.

Publications

Species	Application	Title
human	WB	J Cell Biol; A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER; Authors - Tino Pleiner
human	WB	Elife; The architecture of EMC reveals a path for membrane protein insertion.; Authors - John P O'Donnell
human	WB	bioRxiv; Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins; Authors - Katharine R Page