C16orf84 Polyclonal antibody

C16orf84 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 23337-1-AP

Synonyms

UPF0432, NCS2, Cytosolic thiouridylase subunit 2, Cytoplasmic tRNA 2-thiolation protein 2, CTU2

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 23337-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 23337-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 23337-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 23337-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 23337-1-AP

  IP result of anti-C16orf84 (IP:23337-1-AP, 4ug; Detection:23337-1-AP 1:500) with HepG2 cells lysate 3600ug.

• 

  IHC staining of human kidney using 23337-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 23337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 23337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 23337-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HEK-293 using 23337-1-AP

  Immunofluorescent analysis of HEK-293 cells using 23337-1-AP (C16orf84 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, mouse spleen tissue, Raji cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23337-1-AP targets C16orf84 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C16orf84 fusion protein Ag18428
• Full Name: chromosome 16 open reading frame 84
• Calculated Molecular Weight: 515 aa, 56 kDa
• Observed Molecular Weight: 65-68 kDa
• GenBank Accession Number: BC125269
• Gene Symbol: C16orf84
• Gene ID (NCBI): 348180
• RRID: AB_2879257
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytoplasmic tRNA 2-thiolation protein 2 (CUT2), also named as C16orf84 or NCS2, is a 515 amino acid protein, which belongs to the CUT2 family. C16orf84 localizes in the cytoplasm and Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). C16orf84 may act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thio-carboxylated URM1 onto the uridine of tRNAs at wobble position.