C16orf84 Polyclonal antibody

C16orf84 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 23337-1-AP

Synonyms

UPF0432, NCS2, Cytosolic thiouridylase subunit 2, Cytoplasmic tRNA 2-thiolation protein 2, CTU2



Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, mouse spleen tissue, Raji cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23337-1-AP targets C16orf84 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C16orf84 fusion protein Ag18428
Full Name chromosome 16 open reading frame 84
Calculated Molecular Weight 515 aa, 56 kDa
Observed Molecular Weight 65-68 kDa
GenBank Accession NumberBC125269
Gene Symbol C16orf84
Gene ID (NCBI) 348180
RRIDAB_2879257
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytoplasmic tRNA 2-thiolation protein 2 (CUT2), also named as C16orf84 or NCS2, is a 515 amino acid protein, which belongs to the CUT2 family. C16orf84 localizes in the cytoplasm and Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). C16orf84 may act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thio-carboxylated URM1 onto the uridine of tRNAs at wobble position.

Protocols

Product Specific Protocols
WB protocol for C16orf84 antibody 23337-1-APDownload protocol
IHC protocol for C16orf84 antibody 23337-1-APDownload protocol
IF protocol for C16orf84 antibody 23337-1-APDownload protocol
IP protocol for C16orf84 antibody 23337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols