C16orf93 Polyclonal antibody

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• C16orf93 Polyclonal antibody (25364-1-AP)

C16orf93 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 25364-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

BTBD12, CCDC189, CFAP119, Cilia- and flagella-associated protein 119, Coiled-coil domain-containing protein 189

• 

  WB analysis of Raji using 25364-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25364-1-AP (C16orf93 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HEK-293 using 25364-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using C16orf93 antibody (25364-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 25364-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C16orf93 antibody (25364-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Raji cells
• Positive IF/ICC detected in: HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25364-1-AP targets C16orf93 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21676

  Product name: Recombinant human C16orf93 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-331 aa of BC128129

  Sequence: MLNRKTSHFLGMRVQSELEHLSELRREAGKDRSSVHGSAARTRASVRTQWTTAAAAKADEDPGANLFPPPLPRPRICMWKYLDVHSMHQLEKTTNAEMREVLAELLELGCPEQSLRDAITLDLFCHALIFCRQQGFSLEQTSAACALLQDLHKACIATPLGNVEECYRYFTSVLFCHGVRRPPFSIDLFKEEQLLALEDYVVNTYFRHFKLYKYVFTPQVRLDLSLTYMGLQPPKLWPESETEKEESKEMEEQAVTPQKEELETVAPPEPEPSHIHVLRAYIKTQVNKELEQLQGLVEERLKASEERLSSKLTALERPFQLPPGKGKSKTK

• Full Name: chromosome 16 open reading frame 93
• Calculated Molecular Weight: 331 aa, 38 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC128129
• Gene Symbol: C16orf93
• Gene ID (NCBI): 90835
• RRID: AB_2880045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A1A4V9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C16orf93, also named as CCDC189, is a coiled-coil domain-containing protein which has four isoforms with MW 38 kDa, 34 kDa, 12 kDa and 20 kDa.

Publications

What published studies show

The C16orf93 Polyclonal antibody (Cat# 25364-1-AP) has 2 citations. These appear in World Journal of Men's Health and Andrology, both published in 2026. The associated research fields are reproductive biology and andrology, specifically focusing on male infertility, asthenoteratozoospermia, and sperm flagellar/acrosomal architecture. Both studies utilized the antibody for immunofluorescence (IF) in human samples.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	World J Mens Health; Identification of a Novel Biallelic CFAP119 Variant in an Infertile Man with Asthenoteratozoospermia.; Authors - Jun Ma
human	IF	Andrology; Loss-of-Function Variants in CCDC189 Cause Human Oligoasthenoteratozoospermia by Disrupting Sperm Flagellar and Acrosomal Architecture.; Authors - Jianteng Zhou