C16orf93 Polyclonal antibody

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  WB analysis of Raji using 25364-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25364-1-AP (C16orf93 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 25364-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using C16orf93 antibody (25364-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 25364-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C16orf93 antibody (25364-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

C16orf93 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 25364-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

BTBD12, CCDC189, CFAP119, Cilia- and flagella-associated protein 119, Coiled-coil domain-containing protein 189

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Tested Applications

• Positive WB detected in: Raji cells
• Positive IF/ICC detected in: HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25364-1-AP targets C16orf93 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21676

  Product name: Recombinant human C16orf93 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-331 aa of BC128129

  Sequence: MLNRKTSHFLGMRVQSELEHLSELRREAGKDRSSVHGSAARTRASVRTQWTTAAAAKADEDPGANLFPPPLPRPRICMWKYLDVHSMHQLEKTTNAEMREVLAELLELGCPEQSLRDAITLDLFCHALIFCRQQGFSLEQTSAACALLQDLHKACIATPLGNVEECYRYFTSVLFCHGVRRPPFSIDLFKEEQLLALEDYVVNTYFRHFKLYKYVFTPQVRLDLSLTYMGLQPPKLWPESETEKEESKEMEEQAVTPQKEELETVAPPEPEPSHIHVLRAYIKTQVNKELEQLQGLVEERLKASEERLSSKLTALERPFQLPPGKGKSKTK

• Full Name: chromosome 16 open reading frame 93
• Calculated Molecular Weight: 331 aa, 38 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC128129
• Gene Symbol: C16orf93
• Gene ID (NCBI): 90835
• RRID: AB_2880045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A1A4V9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C16orf93, also named as CCDC189, is a coiled-coil domain-containing protein which has four isoforms with MW 38 kDa, 34 kDa, 12 kDa and 20 kDa.