CTC1 Polyclonal antibody

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  WB analysis using 28268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28268-1-AP (C17orf68 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28268-1-AP

  WB result of C17orf68 antibody (28268-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C17orf68 transfected HeLa cells.

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  IF Staining of mouse brain using 28268-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CTC1 antibody (28268-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

CTC1 Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 28268-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, ELISA

Synonyms

C17orf68, AAF 132, CST complex subunit CTC1, HBV DNAPTP1-transactivated protein B

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, K562 cells
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28268-1-AP targets CTC1 in WB, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTC1 fusion protein Ag28167
• Full Name: chromosome 17 open reading frame 68
• Calculated Molecular Weight: 1217 aa, 135 kDa
• Observed Molecular Weight: 135-150 kDa
• GenBank Accession Number: BC111783
• Gene Symbol: CTC1
• Gene ID (NCBI): 80169
• RRID: AB_2918152
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q2NKJ3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Conserved telomere maintenance component 1 (CTC1) is a subunit of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase, the enzyme that initiates DNA replication (PMID: 19119139). CTC1 also functions in a telomere-associated complex with STN1 and TEN1 (PMID: 19854130).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (02-24-2025)

Antibody doesn't specifically recognize CTC1. A band can still be detected in CTC1 knockdown cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE1