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NCBP3 Polyclonal antibody

NCBP3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24384-1-AP

Synonyms

C17orf85, ELG, HSA277841, NCBP3, Protein ELG

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NCBP3 Polyclonal antibody (24384-1-AP)

    WB analysis using 24384-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24384-1-AP (NCBP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NCBP3 Polyclonal antibody (24384-1-AP)

    IF Staining of HeLa using 24384-1-AP

    Immunofluorescent analysis of HeLa cells using 24384-1-AP (NCBP3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inA549 cells, HeLa cells, Jurkat cells, PC-3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

24384-1-AP targets NCBP3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCBP3 fusion protein Ag20021
Full Name chromosome 17 open reading frame 85
Calculated Molecular Weight 620 aa, 70 kDa
Observed Molecular Weight 71-80 kDa
GenBank Accession NumberBC148856
Gene Symbol NCBP3/C17orf85
Gene ID (NCBI) 55421
RRIDAB_2879518
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

chromosome 17 open reading frame 85(C17orf85)also named as Protein ELG is 620 amino acids protein, which is localizes in human chromosome 17. The function of protein is still non-known and it consists of some phosphorylation site . It may involves in human embryonic stem cell differentiation.

Protocols

Product Specific Protocols
WB protocol for NCBP3 antibody 24384-1-APDownload protocol
IF protocol for NCBP3 antibody 24384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

RNA Biol

NCBP3/SNHG6 inhibits GBX2 transcription in a histone modification manner to facilitate the malignant biological behaviour of glioma cells.

Authors - Xiwen Li
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Hana (Verified Customer) (08-20-2019)

Please make more concentrated Ab.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 5% milk
  • Cell Tissue Type: C2C12 myoblast