C18orf21 Polyclonal antibody

C18orf21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24977-1-AP

Synonyms

XTP13, PNAS-131, PNAS-124, HsT3108, HBV X-transactivated gene 13 protein

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Validation Data Gallery

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  WB analysis of COLO 320 using 24977-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 24977-1-AP (C18orf21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24977-1-AP (C18orf21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 24977-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24977-1-AP (C18orf21 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of COLO 320 using 24977-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 24977-1-AP (C18orf21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: COLO 320 cells
• Positive IHC detected in: human urothelial carcinoma tissue, human colon cancer tissue, human pancreas tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24977-1-AP targets C18orf21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C18orf21 fusion protein Ag21782
• Full Name: chromosome 18 open reading frame 21
• Calculated Molecular Weight: 220 aa, 25 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC119766
• Gene Symbol: C18orf21
• Gene ID (NCBI): 83608
• RRID: AB_2879829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C18orf21, also named as XTP13, belongs to the UPF0711 family. The MW is 25-28 kDa in WB detection.