RMC1 Polyclonal antibody

RMC1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20111-1-AP

Synonyms

MIC1, C18orf8, Mic-1, Mic 1, HsT2591

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Validation Data Gallery

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  WB analysis of human brain using 20111-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20111-1-AP (RMC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 20111-1-AP

  IP result of anti-RMC1 (IP:20111-1-AP, 4ug; Detection:20111-1-AP 1:600) with MCF-7 cells lysate 1120 ug.

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  IHC staining of human colon cancer using 20111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 20111-1-AP (RMC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 20111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 20111-1-AP (C18orf8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon using 20111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20111-1-AP (C18orf8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 20111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20111-1-AP (C18orf8 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human colon cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1500
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20111-1-AP targets RMC1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RMC1 fusion protein Ag13904
• Full Name: chromosome 18 open reading frame 8
• Calculated Molecular Weight: 657 aa, 75 kDa
• Observed Molecular Weight: 75-80 kDa, 66 kDa
• GenBank Accession Number: BC002950
• Gene Symbol: RMC1
• Gene ID (NCBI): 29919
• RRID: AB_10646439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.