MYDGF/C19orf10 Polyclonal antibody

• Featured Product
• KD/KO Validated

MYDGF/C19orf10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 11353-1-AP

Synonyms

MYDGF, C19orf10, UPF0556 protein C19orf10, SF20, Myeloid-derived growth factor

---

Validation Data Gallery

• 

  WB analysis using 11353-1-AP

  various cells were subjected to SDS PAGE followed by western blot with 11353-1-AP (MYDGF/C19orf10F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 11353-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11353-1-AP (MYDGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11353-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11353-1-AP (MYDGF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 11353-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11353-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11353-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF/C19orf10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11353-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11353-1-AP (MYDGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11353-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYDGF/C19orf10 antibody 11353-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 11353-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYDGF/C19orf10 antibody (11353-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Tested Applications

• Positive WB detected in: DU 145 cells, HeLa cells, mouse colon tissue, Jurkat cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Product Information

11353-1-AP targets MYDGF/C19orf10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYDGF/C19orf10 fusion protein Ag1900
• Full Name: chromosome 19 open reading frame 10
• Calculated Molecular Weight: 173 aa, 19 kDa
• Observed Molecular Weight: 15-19 kDa
• GenBank Accession Number: BC010129
• Gene Symbol: MYDGF/C19orf10
• Gene ID (NCBI): 56005
• RRID: AB_2207647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myeloid-derived growth factor (MYDGF), also named as chromosome 19 open reading frame 10 (C19orf10), is an FLS-derived protein that is secreted into the synovial fluid. MYDGF as a paracrine-acting protein secreted from monocytes/macrophages that improves tissue repair and heart function after myocardial infarction. MYDGF has been reported to regulates neutrophil motility in interstitial tissue damage(PMID: 34047769).

Publications

Species	Application	Title
mouse	WB,IF	Sci Adv; Myeloid-derived growth factor inhibits inflammation and alleviates endothelial injury and atherosclerosis in mice.; Authors - Biying Meng ; KO Validated
human,mouse,rat	WB,IHC,IF	Kidney Int; Myeloid-derived Growth Factor Deficiency Exacerbates Mitotic Catastrophe of Podocytes in Glomerular Disease.; Authors - Ping Zhan ; KO Validated
human	WB,IHC	Front Oncol; The Clinical Relevance and Tumor Promoting Function of C19orf10 in Kidney Renal Clear Cell Carcinoma.; Authors - Yanxin Lu ; KD Validated
human	IHC,WB	J Cancer; C19orf10 promotes malignant behaviors of human bladder carcinoma cells via regulating the PI3K/AKT and Wnt/β-catenin pathways.; Authors - Shi Li ; KD Validated
human,mouse	WB,IHC,IF	Cell Death Dis; Myeloid-derived growth factor alleviates non-alcoholic fatty liver disease alleviates in a manner involving IKKβ/NF-κB signaling; Authors - Yan Ding
human	WB	Nat Commun; Identification of myeloid-derived growth factor as a mechanically-induced, growth-promoting angiocrine signal for human hepatocytes; Authors - Linda Große-Segerath