Tested Applications
| Positive WB detected in | MCF-7 cells, HepG2 cells |
| Positive IHC detected in | human kidney tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HaCaT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26338-1-AP targets C19orf21 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23712 Product name: Recombinant human C19orf21 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 330-679 aa of BC052236 Sequence: LLTKLSLITAPRRERGRPSLYVQRDIVQETQREEDHRREGLHVGRASTPDWVSEGPQPGLRRALSSDSILSPAPDARAADPAPEVRKVNRIPPDAYQPYLSPGTPQLEFSAFGAFGKPSSLSTAEAKAATSPKATMSPRHLSESSGKPLSTKQEASKPPRGCPQANRGVVRWEYFRLRPLRFRAPDEPQQAQVPHVWGWEVAGAPALRLQKSQSSDLLERERESVLRREQEVAEERRNALFPEVFSPTPDENSDQNSRSSSQASGITGSYSVSESPFFSPIHLHSNVAWTVEDPVDSAPPGQRKKEQWYAGINPSDGINSEVLEAIRVTRHKNAMAERWESRIYASEEDD Predict reactive species |
| Full Name | chromosome 19 open reading frame 21 |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC052236 |
| Gene Symbol | C19orf21 |
| Gene ID (NCBI) | 126353 |
| RRID | AB_2880482 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IVT2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
C19orf21, also named as MISP, plays a role in mitotic spindle orientation and mitotic progression. It may be required for directed cell migration and centrosome orientation. C19orf21 regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for C19orf21 antibody 26338-1-AP | Download protocol |
| WB protocol for C19orf21 antibody 26338-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MISP antibody (Cat# 26338-1-AP) has 7 citations. These appear in journals including Advanced Science, Cell Death & Disease, International Journal of Molecular Sciences, Frontiers in Pharmacology, Frontiers in Oncology, Discover Oncology, and Journal of Veterinary Medical Science. Research fields span oncology (non-small cell lung cancer, thyroid cancer, colorectal cancer, liver cancer, pancreatic cancer, lung adenocarcinoma), ferroptosis, JAK2-STAT3 and Hippo signaling pathways, and inflammatory bowel disease.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) MISP Suppresses Ferroptosis via MST1/2 Kinases to Facilitate YAP Activation in Non-Small Cell Lung Cancer | ||
Cell Death Discov Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression. | ||
Int J Mol Sci Mitotic Spindle Positioning (MISP) Facilitates Colorectal Cancer Progression by Forming a Complex with Opa Interacting Protein 5 (OIP5) and Activating the JAK2-STAT3 Signaling Pathway
| ||
Front Pharmacol Protein and metabolic profiles of tyrosine kinase inhibitors co-resistant liver cancer cells | ||
Front Oncol Up-Regulated MISP Is Associated With Poor Prognosis and Immune Infiltration in Pancreatic Ductal Adenocarcinoma. | ||
Discov Oncol MISP promotes the progression of lung adenocarcinoma through Inhibition of the Hippo signaling pathway.
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Reviews
What customers say
Both reviewers gave this antibody a 5-star rating. One user confirmed reliable performance in Western Blot (siRNA validated) and Immunofluorescence on Hep2 cells. The other tested it by Western Blot at a 1:1000 dilution across 293T, HaCaT, and U2OS cell lines, reporting the protein appeared at the expected molecular weight with no additional background bands. Overall, users highlight clean specificity, correct band size, and consistent results across multiple applications and cell types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ana (Verified Customer) (04-13-2026) | Works in Western Blot (siRNA validated) and in Immunofluorescence
 |
anwesh (Verified Customer) (07-29-2019) | The protein was found at the right size and no additional background bands were observed around the size range. Perfect!
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