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C19orf43 Polyclonal antibody

C19orf43 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19420-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

TRIR, EC:3.1.13.-, Exoribonuclease TRIR, fSAP18, Telomerase RNA component interacting RNase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of A431 using 19420-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 19420-1-AP (C19orf43 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 19420-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 19420-1-AP (C19orf43 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 19420-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19420-1-AP (C19orf43 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human gliomas using 19420-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19420-1-AP (C19orf43 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 19420-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19420-1-AP (C19orf43 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat colon using 19420-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 19420-1-AP (C19orf43 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse colon using 19420-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 19420-1-AP (C19orf43 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A431 cells, HEK-293 cells
Positive IHC detected in	human colon tissue, human gliomas tissue, human stomach cancer tissue, mouse colon tissue, rat colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19420-1-AP targets C19orf43 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13747 Product name: Recombinant human C19orf43 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-176 aa of BC000216 Sequence: MAARGRRAEPQGREAPGPAGGGGGGSRWAESGSGTSPESGDEEVSGAGSSPVSGGVNLFANDGSFLELFKRKMEEEQRQRQEEPPPGPQRPDQSAAAAGPGDPKRKGGPGSTLSFVGKRRGGNKLALKTGIVAKKQKTEDEVLTSKGDAWAKYMAEVKKYKAHQCGDDDKTRPLVK Predict reactive species
Full Name	chromosome 19 open reading frame 43
Calculated Molecular Weight	176 aa, 18 kDa
Observed Molecular Weight	28 kDa
GenBank Accession Number	BC000216
Gene Symbol	C19orf43
Gene ID (NCBI)	79002
RRID	AB_10640586
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BQ61
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C19orf43, also named as MGC2803 and fSAP18, is an Uncharacterized protein. It is 176aa,18.4kd. We used the antibody raised from the C19orf43 protein, we get a ~28kd in human placenta, 293 and A431 cell.

Protocols

Product Specific Protocols
IHC protocol for C19orf43 antibody 19420-1-AP	Download protocol
WB protocol for C19orf43 antibody 19420-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The C19orf43 Polyclonal antibody (Cat# 19420-1-AP) has 1 total citation. It was cited in Cell Reports (2023), in a study investigating how TERRA R-loops connect and protect sister telomeres during mitosis. The research field encompasses telomere biology, chromosome structure, and cell division. The antibody was used for Western blotting and immunoprecipitation in human samples.