Tested Applications
| Positive WB detected in | DU 145 cells |
| Positive IP detected in | DU 145 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10711-1-AP targets C1D in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1121 Product name: Recombinant human C1D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-141 aa of BC005235 Sequence: MAGEEINEDYPVEIHEYLSAFENSIGAVDEMLKTMMSVSRNELLQKLDPLEQAKVDLVSAYTLNSMFWVYLATQGVNPKEHPVKQELERIRVYMNRVKEITDKKKAGKLDRGAASRFVKNALWEPKSKNASKVANKGKSKS Predict reactive species |
| Full Name | C1D nuclear receptor co-repressor |
| Calculated Molecular Weight | 16 kDa |
| GenBank Accession Number | BC005235 |
| Gene Symbol | C1D |
| Gene ID (NCBI) | 10438 |
| RRID | AB_2067014 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13901 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for C1D antibody 10711-1-AP | Download protocol |
| IP protocol for C1D antibody 10711-1-AP | Download protocol |
| WB protocol for C1D antibody 10711-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The C1D Polyclonal antibody (catalog no. 10711-1-AP) has 2 citations. These appear in Redox Biology and EMBO Journal. The cited research spans two fields: one study on multi-omics profiling of chitinase-3-like protein 1 as a mediator in sarcopenia–liver cancer crosstalk (using WB in mouse), and another on the cellular assembly and functional resilience of the mammalian RNA exosome.
| Species | Application | Title |
|---|---|---|
Redox Biol Multi-omics profiling reveals Chitinase-3-like protein 1 as a key mediator in the crosstalk between sarcopenia and liver cancer | ||
Reviews
What customers say
The single available review rates this antibody 4 out of 5 stars for Western Blot. The reviewer used a 1:200 dilution with 10 µg of total mouse protein loaded per lane and noted that the antibody also stains the protein ladder, which may be relevant for interpreting results. Overall, the feedback suggests the antibody performs well for WB on mouse samples, though users should be aware of potential non-specific binding to ladder components.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ralf (Verified Customer) (08-04-2022) | 10µg of total mouse protein were loaded on the gel. Antibodies also stain the ladder.
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