CTRP1 Polyclonal antibody

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  WB analysis of human testis using 12209-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12209-1-AP (CTRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12209-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12209-1-AP (CTRP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 12209-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 12209-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 12209-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 12209-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 12209-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12209-1-AP (CTRP1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12209-1-AP

  Immunofluorescent analysis of HepG2 cells, using C1QTNF1 antibody 12209-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

CTRP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12209-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C1QTNF1, UNQ310/PRO353, GIP, G protein-coupled receptor-interacting protein, Complement C1q tumor necrosis factor-related protein 1

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Tested Applications

• Positive WB detected in: human testis tissue, J774A.1 cells, RAW 264.7 cells
• Positive IHC detected in: human prostate cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

12209-1-AP targets CTRP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2854

  Product name: Recombinant human C1QTNF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-281 aa of BC021553

  Sequence: MGSRGQGLLLAYCLLLAFASGLVLSRVPHVQGEQQEWEGTEELPSPPDHAERAEEQHEKYRPSQDQGLPASRCLRCCDPGTSMYPATAVPQINITILKGEKGDRGDRGLQGKYGKTGSAGARGHTGPKGQKGSMGAPGERCKSHYAAFSVGRKKPMHSNHYYQTVIFDTEFVNLYDHFNMFTGKFYCYVPGLYFFSLNVHTWNQKETYLHIMKNEEEVVILFAQVGDRSIMQSQSLMLELREQDQVWVRLYKGERENAIFSEELDTYITFSGYLVKHATEP

• Full Name: C1q and tumor necrosis factor related protein 1
• Calculated Molecular Weight: 281 aa, 32 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC021553
• Gene Symbol: CTRP1
• Gene ID (NCBI): 114897
• RRID: AB_10644328
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BXJ1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C1q and tumor necrosis factor related protein 1(CTRP1), also known as C1QTNF1, is a member of the CTRP superfamily. CTRP1 is a secreted glycoprotein synthesized by a variety of tissues and cells, such as adipose tissue and macrophages. CTRP1 is a paralog of adiponectin. CTRP1 plays a critical role in regulating inflammation and lipid metabolism and acts as a targeted treatment for atherosclerosis, heart failure, hypertension, NAFLD, and diabetes mellitus (PMID: 36812667).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Front Cell Dev Biol; CTRP1 Attenuates Cerebral Ischemia/Reperfusion Injury via the PERK Signaling Pathway.; Authors - Huizhi Fei ; KD Validated
human	WB	Biomed Res Int; Identification of CTRP1 as a Prognostic Biomarker and Oncogene in Human Glioblastoma.; Authors - Liyan Chen ; KD Validated
human	IHC	Front Mol Biosci; High C1QTNF1 expression mediated by potential ncRNAs is associated with poor prognosis and tumor immunity in kidney renal clear cell carcinoma; Authors - Jiechuan Qiu