CTRP4 Polyclonal antibody

CTRP4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14023-1-AP

Synonyms

C1QTNF4, CTRP4, ZACRP4

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Validation Data Gallery

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  WB analysis of BxPC-3 using 14023-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14023-1-AP (CTRP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 14023-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14023-1-AP (CTRP4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 14023-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14023-1-AP (CTRP4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of BxPC-3 using 14023-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 14023-1-AP (CTRP4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: BxPC-3 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

14023-1-AP targets CTRP4 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTRP4 fusion protein Ag5147
• Full Name: C1q and tumor necrosis factor related protein 4
• Calculated Molecular Weight: 329 aa, 35 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC035628
• Gene Symbol: C1QTNF4
• Gene ID (NCBI): 114900
• RRID: AB_10644162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Ann Transl Med; C1Q/TNF-related protein 4 expression correlates with herpes simplex encephalitis progression.; Authors - Wangshu Xu