Tested Applications
| Positive WB detected in | human plasma, human blood |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17346-1-AP targets C1r in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11390 Product name: Recombinant human C1R protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 356-705 aa of BC035220 Sequence: AVCQDDGTWHRAMPRCKIKDCGQPRNLPNGDFRYTTTMGVNTYKARIQYYCHEPYYKMQTRAGSRESEQGVYTCTAQGIWKNEQKGEKIPRCLPVCGKPVNPVEQRQRIIGGQKAKMGNFPWQVFTNIHGRGGGALLGDRWILTAAHTLYPKEHEAQSNASLDVFLGHTNVEELMKLGNHPIRRVSVHPDYRQDESYNFEGDIALLELENSVTLGPNLLPICLPDNDTFYDLGLMGYVSGFGVMEEKIAHDLRFVRLPVANPQACENWLRGKNRMDVFSQNMFCAGHPSLKQDACQGDSGGVFAVRDPNTDRWVATGIVSWGIGCSRGYGFYTKVLNYVDWIKKEMEEED Predict reactive species |
| Full Name | complement component 1, r subcomponent |
| Calculated Molecular Weight | 705 aa, 80 kDa |
| Observed Molecular Weight | 85 kDa, 30 kDa |
| GenBank Accession Number | BC035220 |
| Gene Symbol | C1R |
| Gene ID (NCBI) | 715 |
| RRID | AB_2878392 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00736 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r acts by transforming the activation signal into an enzymic activity. C1r is a single-chain glycoprotein that can be cleaved into an A chain and a B chain upon activation. This antibody recognizes the full-length C1r chain and the cleaved B chain.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for C1r antibody 17346-1-AP | Download protocol |
| WB protocol for C1r antibody 17346-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The C1r Polyclonal antibody (Cat# 17346-1-AP) has 3 total citations. These appear in Cell Reports in Medicine (2025), Scientific Reports (2020), and Molecular Carcinogenesis (2024). The associated research fields include glioblastoma genomic and immunological profiling, complement component C1r's role in CRP-mediated uric acid crystal recognition, and C1r downregulation in hepatocellular carcinoma via HIF-1α-regulated glycolysis. Applications cited include WB, IP, and IHC.
| Species | Application | Title |
|---|---|---|
Cell Rep Med Genomic landscape and immunological profile of glioblastoma in East Asian patients | ||
Sci Rep C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1. | ||
Mol Carcinog Downregulation of C1R promotes hepatocellular carcinoma development by activating HIF-1α-regulated glycolysis |
