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C1R Polyclonal antibody

C1R Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17346-1-AP

Synonyms

C1R, Complement C1r subcomponent

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human blood using C1R Polyclonal antibody (17346-1-AP)

    WB analysis of human blood using 17346-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 17346-1-AP (C1R Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using C1R Polyclonal antibody (17346-1-AP)

    IHC staining of human liver using 17346-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17346-1-AP (C1R Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using C1R Polyclonal antibody (17346-1-AP)

    IHC staining of human liver using 17346-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17346-1-AP (C1R Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inhuman blood tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 17346-1-AP is C1R Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1R fusion protein Ag11390
Full Name complement component 1, r subcomponent
Calculated Molecular Weight 705 aa, 80 kDa
Observed Molecular Weight 85 kDa, 30 kDa
GenBank Accession NumberBC035220
Gene Symbol C1R
Gene ID (NCBI) 715
RRIDAB_2878392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r acts by transforming the activation signal into an enzymic activity. C1r is a single-chain glycoprotein which can be cleaved into an A chain and a B chain upon activation. This antibody recognizes full-length C1r chain and the cleaved B chain.

Protocols

Product Specific Protocols
WB protocol for C1R antibody 17346-1-APDownload protocol
IHC protocol for C1R antibody 17346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.

Authors - Anika Alberts
View Article