METTL18 Polyclonal antibody

METTL18 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25553-1-AP

Synonyms

C1orf156, Methyltransferase-like protein 18, Histidine protein methyltransferase 1 homolog, EC:2.1.1.85, AsTP2

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Validation Data Gallery

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  WB analysis of A431 using 25553-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25553-1-AP (C1orf156 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 25553-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25553-1-AP (C1orf156 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 25553-1-AP

  Immunofluorescent analysis of A431 cells using 25553-1-AP (C1orf156 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, PC-3 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

25553-1-AP targets METTL18 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: METTL18 fusion protein Ag22148
• Full Name: chromosome 1 open reading frame 156
• Calculated Molecular Weight: 372 aa, 42 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC008679
• Gene Symbol: METTL18
• Gene ID (NCBI): 92342
• RRID: AB_2880129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL18 belongs to a family of protein lysine methyltransferases that methylate nonhistone proteins. Methyltransferases catalyze the transfer of a methyl group from a donor, generally S-adenosyl-L-methionine, to acceptor molecules, including proteins, DNA, RNA, lipids, and small metabolites. METTL18 is involved in peptidyl-lysine monomethylation.

Publications

Species	Application	Title
human	WB	Elife; METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.; Authors - Eriko Matsuura-Suzuki
human	WB	Front Oncol; Identification METTL18 as a Potential Prognosis Biomarker and Associated With Immune Infiltrates in Hepatocellular Carcinoma.; Authors - Tian-Hao Li