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C1orf83 Recombinant monoclonal antibody, PBS Only (83626-3-PBS)

Filter: AllIHCIF/ICCFC (Intra)Affinity

IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 83626-3-RR (same clone as 83626-3-PBS)
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C1orf83 antibody (83626-3-RR, Clone: 240678D10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.
FC experiment of U2OS using 83626-3-RR (same clone as 83626-3-PBS)
1x10^6 U2OS cells were intracellularly stained with 0.25 ug C1orf83 Recombinant antibody (83626-3-RR, Clone:240678D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.
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1x10^6 U2OS cells were intracellularly stained with 0.25 ug C1orf83 Recombinant antibody (83626-3-RR, Clone:240678D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83626-3-RR
Biolayer interferometry (BLl) kinetic assays of 83626-3-RR against Human C1orf83 were performed. The affinity constant is below 1 pM.

C1orf83 Recombinant monoclonal antibody, PBS Only

Name: C1orf83 Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 83626-3-PBS

C1orf83 Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 83626-3-PBS

Clone No.240678D10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

240678D10, FLJ32112, FLJ39169, RP4 758J24.3, TCEANC2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

83626-3-PBS targets C1orf83 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag15040 Product name: Recombinant human C1orf83 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-208 aa of BC035374 Sequence: MDKFVIRTPRIQNSPQKKDSGGKVYKQATIESLKRVVVVEDIKRWKTMLELPDQTKENLVEALQELKKKIPSREVLKSTRIGHTVNKMRKHSDSEVASLAREVYTEWKTFTEKHSNRPSIEVRSDPKTESLRKNAQKLLSEALELKMDHLLVENIERETFHLCSRLINGPYRRTVRALVFTLKHRAEIRAQVKSGSLPVGTFVQTHKK Predict reactive species
Full Name	chromosome 1 open reading frame 83
Calculated Molecular Weight	208 aa, 23 kDa
GenBank Accession Number	BC035374
Gene Symbol	C1orf83
Gene ID (NCBI)	127428
RRID	AB_3671238
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q96MN5
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

C1orf83, also known as TCEANC2, and the rs10788972 variant of the TCEANC2 gene in the PARK10 region were identified as being strongly associated with sporadic PD in the US population.