C1orf83 Recombinant monoclonal antibody, PBS Only (Detector)

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  IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 83626-3-RR (same clone as 83626-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C1orf83 antibody (83626-3-RR, Clone: 240678D10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83626-3-RR (same clone as 83626-3-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug C1orf83 Recombinant antibody (83626-3-RR, Clone:240678D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00578-2

  Cytometric bead array standard curve of MP00578-2, C1orf83 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83626-1-PBS. Detection antibody: 83626-3-PBS. Standard: Ag15040. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83626-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83626-3-RR against Human C1orf83 were performed. The affinity constant is below 1 pM.

C1orf83 Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83626-3-PBS

Clone No.240678D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

240678D10, FLJ32112, FLJ39169, RP4 758J24.3, TCEANC2

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Product Information

83626-3-PBS targets C1orf83 as part of a matched antibody pair:

MP00578-2: 83626-1-PBS capture and 83626-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag15040

  Product name: Recombinant human C1orf83 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-208 aa of BC035374

  Sequence: MDKFVIRTPRIQNSPQKKDSGGKVYKQATIESLKRVVVVEDIKRWKTMLELPDQTKENLVEALQELKKKIPSREVLKSTRIGHTVNKMRKHSDSEVASLAREVYTEWKTFTEKHSNRPSIEVRSDPKTESLRKNAQKLLSEALELKMDHLLVENIERETFHLCSRLINGPYRRTVRALVFTLKHRAEIRAQVKSGSLPVGTFVQTHKK

• Full Name: chromosome 1 open reading frame 83
• Calculated Molecular Weight: 208 aa, 23 kDa
• GenBank Accession Number: BC035374
• Gene Symbol: C1orf83
• Gene ID (NCBI): 127428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96MN5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.