C2 Polyclonal antibody

C2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC

Conjugate

Unconjugated

Publications

2

Cat no : 27175-1-AP

Synonyms

C2, C3/C5 convertase, CO2, Complement C2, complement component 2

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Validation Data Gallery

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  WB analysis of A431 using 27175-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 27175-1-AP (C2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27175-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27175-1-AP (C2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 27175-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27175-1-AP (C2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 27175-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27175-1-AP (C2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 27175-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27175-1-AP (C2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, Raji cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 27175-1-AP is C2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C2 fusion protein Ag25690
• Full Name: complement component 2
• Observed molecular weight: 70 kDa, 80 kDa
• GenBank accession number: BC043484
• Gene symbol: C2
• Gene ID (NCBI): 717
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Mol Gastroenterol Hepatol; A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.; Authors - Dayan Sun
	IHC	Insect Biochem Mol Biol; Sequence analysis and function of mosquito aeCCC2 and Drosophila Ncc83 orthologs.; Authors - Phu C Duong