C20orf195 Polyclonal antibody

C20orf195 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16040-1-AP

Synonyms

FNDC11, Fibronectin type III domain-containing protein 11

---

Validation Data Gallery

• 

  WB analysis of SMMC-7721 using 16040-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16040-1-AP (C20orf195 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16040-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16040-1-AP (C20orf195 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IHC staining of human prostate cancer using 16040-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16040-1-AP (C20orf195 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 16040-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16040-1-AP (C20orf195 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16040-1-AP

  Immunofluorescent analysis of HepG2 cells using 16040-1-AP (C20orf195 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HepG2 cells, SMMC-7721 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16040-1-AP targets C20orf195 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C20orf195 fusion protein Ag8914
• Full Name: chromosome 20 open reading frame 195
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC000912
• Gene Symbol: C20orf195
• Gene ID (NCBI): 79025
• RRID: AB_2878209
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.