RTF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RTF2 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16633-1-AP

Synonyms

C20orf43, CDA05, CDAO5, HSPC164, HSPC169, RTF2, SHUJUN 3, UPF0549 protein C20orf43

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Validation Data Gallery

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  WB analysis using 16633-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16633-1-AP (RTF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16633-1-AP

  WB result of RTF2 antibody (16633-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RTF2 transfected HeLa cells.

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  IP experiment of HeLa using 16633-1-AP

  IP result of anti-RTF2 (IP:16633-1-AP, 3ug; Detection:16633-1-AP 1:500) with HeLa cells lysate 3440ug.

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  IF Staining of HeLa using 16633-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16633-1-AP (C20orf43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below

Product Information

16633-1-AP targets RTF2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RTF2 fusion protein Ag9972
• Full Name: chromosome 20 open reading frame 43
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC003359
• Gene Symbol: C20orf43
• Gene ID (NCBI): 51507
• RRID: AB_2256547
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of C20orf43 remains largely unknown. Catalog#16633-1-AP is a rabbit polyclonal antibody raised against the full-length of human C20orf43. The MW of this protein is 40 kDa, and this antibody specially recognises the 40 kDa protein.

Publications

Species	Application	Title
human	WB	Mol Cell; Removal of RTF2 from Stalled Replisomes Promotes Maintenance of Genome Integrity.; Authors - Molly C Kottemann ; KD Validated
human,mouse	WB	bioRxiv; RTF2 controls replication repriming and ribonucleotide excision at the replisome; Authors - Brooke A Conti ; KD Validated