• Featured Product
  • KD/KO Validated

RtcB-Specific Polyclonal antibody

RtcB-Specific Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 19809-1-AP

Synonyms

C22orf28, FAAP, HSPC117, RtcB



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, Jurkat cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19809-1-AP is RtcB-Specific Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RtcB-Specific fusion protein Ag13836
Full Name chromosome 22 open reading frame 28
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC000151
Gene symbol C22orf28
Gene ID (NCBI) 51493
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RTCB (also known as HSPC117, C22orf28, FAAP and D10Wsu52e) is an essential subunit of a tRNA ligase complex that is involved in RNA repair and stress-induced splicing. As a multifunctional protein, RTCB is also a cell adhesion protein and is important in embryo and placenta development. Recently RTCB has been identified as UPR RNA ligase catalyzing unconventional XBP1 mRNA splicing. This antibody specifically recognizes endogenous RTCB protein. (25087875)

Protocols

Product Specific Protocols
WB protocol for RtcB-Specific antibody 19809-1-APDownload protocol
IF protocol for RtcB-Specific antibody 19809-1-APDownload protocol
IP protocol for RtcB-Specific antibody 19809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress.

Authors - Irem Unlu
  • KO Validated
humanWB,IP

Mol Cell

A Synthetic Biology Approach Identifies the Mammalian UPR RNA Ligase RtcB.

Authors - Yanyan Lu