|Positive WB detected in||HeLa cells, mouse brain tissue, Jurkat cells|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 19809-1-AP is RtcB-Specific Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RtcB-Specific fusion protein Ag13836|
|Full Name||chromosome 22 open reading frame 28|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC000151|
|Gene ID (NCBI)||51493|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RTCB (also known as HSPC117, C22orf28, FAAP and D10Wsu52e) is an essential subunit of a tRNA ligase complex that is involved in RNA repair and stress-induced splicing. As a multifunctional protein, RTCB is also a cell adhesion protein and is important in embryo and placenta development. Recently RTCB has been identified as UPR RNA ligase catalyzing unconventional XBP1 mRNA splicing. This antibody specifically recognizes endogenous RTCB protein. (25087875)
J Biol Chem
The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress.
A Synthetic Biology Approach Identifies the Mammalian UPR RNA Ligase RtcB.