C2orf67 Polyclonal antibody

C2orf67 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24183-1-AP

Synonyms

C2orf67, KANSL1L

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Validation Data Gallery

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  WB analysis using 24183-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24183-1-AP (C2orf67 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 24183-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24183-1-AP (C2orf67 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 24183-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24183-1-AP (C2orf67 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 24183-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24183-1-AP (C2orf67 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 24183-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24183-1-AP (C2orf67 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A549 using 24183-1-AP

  Immunofluorescent analysis of A549 cells using 24183-1-AP (C2orf67 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: human testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24183-1-AP is C2orf67 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C2orf67 fusion protein Ag21471
• Full Name: chromosome 2 open reading frame 67
• Calculated Molecular Weight: 987 aa, 112 kDa
• Observed Molecular Weight: 112 kDa, 80 kDa
• GenBank Accession Number: BC126155
• Gene Symbol: C2orf67
• Gene ID (NCBI): 151050
• RRID: AB_2879448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C2orf67,also termed as chromosome 2 open reading frame 67, encodes a 705 amino-acid protein. It exists a variant, which encodes 987 amino-acid protein. The C2orf56 may express in lymphoid /immune and nervous system. The specific function of C2orf67 is still non-known.