C4-B Alpha Chain/C4b-B/C4d-B Polyclonal antibody

C4-B Alpha Chain/C4b-B/C4d-B Polyclonal Antibody for WB, ELISA
Cat No. 55228-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

C4, C4B, C4B1, C4B12, C4B2, C4B3, C4B5, C4F, CH, CO4, Complement C4-B, CPAMD3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inhuman blood

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55228-1-AP targets C4-B Alpha Chain/C4b-B/C4d-B in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide Predict reactive species
Full Name complement component 4B (Chido blood group)
Calculated Molecular Weight 193 kDa
Observed Molecular Weight 95 kDa, 300 kDa
GenBank Accession NumberNM_000592
Gene Symbol C4B
Gene ID (NCBI) 721
RRIDAB_2881291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0C0L5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1116-1128aa of human C4-B protein can recognize C4-B precursor, C4-B alpha chain, C4b-B, and C4d-B.

Protocols

Product Specific Protocols
WB protocol for C4-B Alpha Chain/C4b-B/C4d-B antibody 55228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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