C4BPB Recombinant antibody

• 

  WB analysis using 85812-4-RR

  Human plasma sample was subjected to SDS PAGE followed by western blot with 85812-4-RR (C4BPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

C4BPB Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85812-4-RR

Clone No.250135C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C4b binding protein beta chain, C4b-binding protein beta chain, C4BP

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Tested Applications

• Positive WB detected in: human plasma

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85812-4-RR targets C4BPB in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: C4BPB fusion protein Eg2976
• Full Name: complement component 4 binding protein, beta
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: NM_000716.3
• Gene Symbol: C4BPB
• Gene ID (NCBI): 725
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20851-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C4b-binding protein (C4BP) is a large oligomeric glycoprotein, present in plasma at a concentration of approximately 200 mg/L (PMID: 24218259). It is the main inhibitor of the classical complement and lectin pathway. C4BP is composed of two polypeptides (alpha and beta chains), which have molecular weights of 70 and 45 kDa, respectively (PMID: 7561113).