C4b Alpha Chain/C4b-B/C4d-B Recombinant monoclonal antibody

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• C4b Alpha Chain/C4b-B/C4d-B Recombinant monoclonal antibody (85782-2-RR)

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  WB analysis of human blood using 85782-2-RR

  Human blood was subjected to SDS PAGE followed by western blot with 85782-2-RR (C4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85782-2-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using C4B antibody (85782-2-RR, Clone: 250030C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C4b Alpha Chain/C4b-B/C4d-B Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85782-2-RR

Clone No.250030C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

C4B, C4, C4B1, C4B12, C4B2

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Tested Applications

• Positive WB detected in: human blood
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85782-2-RR targets C4b Alpha Chain/C4b-B/C4d-B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: complement component 4B (Chido blood group)
• Calculated Molecular Weight: 193 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: NM_000592
• Gene Symbol: C4B
• Gene ID (NCBI): 721
• RRID: AB_3744369
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P0C0L5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single-chain precursor, which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular weights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1116-1128aa of human C4b protein can recognize C4b precursor, C4b alpha chain, C4b-B, and C4d-B.