HEK-293 cells were subjected to SDS PAGE followed by western blot with 24319-1-AP (C5orf24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24319-1-AP (C5orf24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 24319-1-AP
IP result of anti-C5orf24 (IP:24319-1-AP, 4ug; Detection:24319-1-AP 1:1000) with HEK-293 cells lysate 3200ug.
IP result of anti-C5orf24 (IP:24319-1-AP, 4ug; Detection:24319-1-AP 1:1000) with HEK-293 cells lysate 3200ug.
IHC staining of human pancreas using 24319-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 24319-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 24319-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 24319-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 24319-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C5orf24 antibody (24319-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C5orf24 antibody (24319-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of HEK-293 using 24319-1-AP
1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug C5orf24 Polyclonal antibody (24319-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug C5orf24 Polyclonal antibody (24319-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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human pancreas tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HEK-293 cells
Positive FC (Intra) detected in
HEK-293 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
C5orf24 is a cellular transcriptional regulator that performs different functions in different cell types, including the processes of cell proliferation, cell polarization, migration, and tumor development.C5orf24 is able to specifically and efficiently capture a wide range of tuberculosis antigens, which is important for the diagnosis of tuberculosis. C5orf24 has two isoforms with MW 20 kDa and 16 kDa. For phosphoserine modification, the MW is about 20-23 kDa.
Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24319-1-AP (C5orf24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human pancreas using 24319-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 24319-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 24319-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 24319-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HEK-293 using 24319-1-AP
IP result of anti-C5orf24 (IP:24319-1-AP, 4ug; Detection:24319-1-AP 1:1000) with HEK-293 cells lysate 3200ug.
IF/ICC Figures
IF Staining of HEK-293 using 24319-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C5orf24 antibody (24319-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of HEK-293 using 24319-1-AP
1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug C5orf24 Polyclonal antibody (24319-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
C5orf24 Polyclonal antibody
Catalog Number
24319-1-AP
Citations
1
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Reactivity
human, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
