CEP85L Polyclonal antibody

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CEP85L Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24588-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

C6orf204, Serologically defined breast cancer antigen NY-BR-15, NY-BR-15, NY BR 15, Centrosomal protein of 85 kDa-like

• 

  WB analysis of PC-13 using 24588-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 24588-1-AP (C6orf204 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 24588-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24588-1-AP (CEP85L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 24588-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24588-1-AP (CEP85L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: PC-13 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:800
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24588-1-AP targets CEP85L in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20235

  Product name: Recombinant human C6orf204 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 146-496 aa of BC153054

  Sequence: DMKDFRPLRKWSSLSKLTAPDNCGQGGTVCREESRNGLEKIGKAKALTSQLRTIGPSCLHDSMEMLRLEDKEINKKRSSTLDCKYKFESCSKEDFRASSSTLRRQPVDMTYSALPESKPIMTSSEAFEPPKYLMLGQQAVGGVPIQPSVRTQMWLTEQLRTNPLEGRNTEDSYSLAPWQQQQIEDFRQGSETPMQVLTGSSRQSYSPGYQDFSKWESMLKIKEGLLRQKEIVIDRQKQQITHLHERIRDNELRAQHAMLGHYVNCEDSYVASLQPQYENTSLQTPFSEESVSHSQQGEFEQKLASTEKEVLQLNEFLKQRLSLFSEEKKKLEEKVGFSNKVELGQQHFLSI

• Full Name: chromosome 6 open reading frame 204
• Calculated Molecular Weight: 805 aa, 92 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC153054
• Gene Symbol: CEP85L
• Gene ID (NCBI): 387119
• RRID: AB_2819012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5SZL2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP85L, also named as C60rf204, is a gene which encodes a protein that has identified as a breast cancer antigen. Nothing more is known of its function at this time. There're five transcript variants with MW 45 kDa, 55-56 kDa and 91-92 kDa.

Publications

What published studies show

The anti-CEP85L antibody (Cat# 24588-1-AP) has 2 total citations, both published in Neuron (IF 16.9, 2020). The associated research fields include neuronal migration regulation in the posterior neocortex and pathogenic variants causing posterior predominant lissencephaly, spanning human and mouse studies with applications in WB, IF, and CoIP.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF,CoIP	Neuron; Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.; Authors - Andrew Kodani ; KD Validated
mouse	WB,IF	Neuron; Pathogenic Variants in CEP85L Cause Sporadic and Familial Posterior Predominant Lissencephaly.; Authors - Meng-Han Tsai