C7orf50 Polyclonal antibody

C7orf50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20797-1-AP

Synonyms

YCR016W, FP15621

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Validation Data Gallery

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  WB analysis using 20797-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20797-1-AP (C7orf50 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 20797-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 20797-1-AP (C7orf50 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 20797-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 20797-1-AP (C7orf50 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of L02 using 20797-1-AP

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using 20797-1-AP (C7orf50 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, Jurkat cells, L02 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

20797-1-AP targets C7orf50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C7orf50 fusion protein Ag14709
• Full Name: chromosome 7 open reading frame 50
• Calculated Molecular Weight: 194 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC006224
• Gene Symbol: C7orf50
• Gene ID (NCBI): 84310
• RRID: AB_10732839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C7orf50 (Chromosome 7, Open Reading Frame 50) is ubiquitously expressed in the kidneys, brain, fat, prostate, spleen, among 22 other tissues and demonstrates low tissue specificity.

Publications

Species	Application	Title
human	WB	Mol Cell; Phosphorylation of the Ribosomal Protein RPL12/uL11 Affects Translation during Mitosis.; Authors - Koshi Imami
human	WB	Life Sci Alliance; The nucleolar phase of signal recognition particle assembly; Authors - Amani Issa