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C8A Polyclonal antibody

C8A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25289-1-AP

Synonyms

C8A, Complement component 8 subunit alpha, Complement component C8 alpha chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C8A Polyclonal antibody (25289-1-AP)

    WB analysis using 25289-1-AP

    Human plasma were subjected to SDS PAGE followed by western blot with 25289-1-AP (C8A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25289-1-AP targets C8A in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C8A fusion protein Ag18389
Full Name complement component 8, alpha polypeptide
Calculated Molecular Weight 584 aa, 65 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC132913
Gene Symbol C8A
Gene ID (NCBI) 731
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C8 is a component of the complement system and contains three polypeptides, alpha, beta and gamma. Complement component C8 alpha chain (C8A) is a constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself. Mutations in the C8A gene cause complement C8 alpha-gamma deficiency.

Protocols

Product Specific Protocols
WB protocol for C8A antibody 25289-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols