C9 Recombinant monoclonal antibody, PBS Only

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• C9 Recombinant monoclonal antibody, PBS Only (84890-4-PBS)

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  WB analysis of human placenta using 84890-4-RR (same clone as 84890-4-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84890-4-RR (C9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84890-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84890-4-RR (same clone as 84890-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84890-4-RR (C9 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84890-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 84890-4-RR (same clone as 84890-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84890-4-RR (C9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84890-4-PBS in a different storage buffer formulation.

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  Affinity of 84890-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84890-4-RR against Human C9 were performed. The affinity constant is 0.739 nM.

C9 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84890-4-PBS

Clone No.242475B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Complement C9, complement component 9, Complement component C9, Complement component C9a, Complement component C9b

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Product Information

84890-4-PBS targets C9 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1282

  Product name: Recombinant Human Component C9 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 22-559 aa of NM_001737.4

  Sequence: QYTTSYDPELTESSGSASHIDCRMSPWSEWSQCDPCLRQMFRSRSIEVFGQFNGKRCTDAVGDRRQCVPTEPCEDAEDDCGNDFQCSTGRCIKMRLRCNGDNDCGDFSDEDDCESEPRPPCRDRVVEESELARTAGYGINILGMDPLSTPFDNEFYNGLCNRDRDGNTLTYYRRPWNVASLIYETKGEKNFRTEHYEEQIEAFKSIIQEKTSNFNAAISLKFTPTETNKAEQCCEETASSISLHGKGSFRFSYSKNETYQLFLSYSSKKEKMFLHVKGEIHLGRFVMRNRDVVLTTTFVDDIKALPTTYEKGEYFAFLETYGTHYSSSGSLGGLYELIYVLDKASMKRKGVELKDIKRCLGYHLDVSLAFSEISVGAEFNKDDCVKRGEGRAVNITSENLIDDVVSLIRGGTRKYAFELKEKLLRGTVIDVTDFVNWASSINDAPVLISQKLSPIYNLVPVKMKNAHLKKQNLERAIEDYINEFSVRKCHTCQNGGTVILMDGKCLCACPFKFEGIACEISKQKISEGLPALEFPNEK

• Full Name: complement component 9
• Calculated Molecular Weight: 63kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: NM_001737.4
• Gene Symbol: C9
• Gene ID (NCBI): 735
• RRID: AB_3743814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P02748
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The final consequence of complement activation is the formation of the membrane attack complex (MAC), a multiprotein assembly that perforates cell membranes, forming transmembrane channels. The MAC plays a critical role in complement-mediated hemolysis and inflammasome activation (PMID: 27444648). Complement component 9 (C9) is 1 of 5 late-acting proteins of complement that participate in the assembly and function of the MAC. C9 may be cleaved by thrombin to generate fragments C9a (25 kDa) and C9b (37 kDa) (PMID: 7523406).