C9 Recombinant monoclonal antibody

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• C9 Recombinant monoclonal antibody (84890-4-RR)

• 

  WB analysis of human placenta using 84890-4-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84890-4-RR (C9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 84890-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84890-4-RR (C9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 84890-4-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84890-4-RR (C9 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity of 84890-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84890-4-RR against Human C9 were performed. The affinity constant is 0.739 nM.

C9 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 84890-4-RR

Clone No.242475B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Complement C9, complement component 9, Complement component C9, Complement component C9a, Complement component C9b

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human colon tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84890-4-RR targets C9 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1282

  Product name: Recombinant Human Component C9 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 22-559 aa of NM_001737.4

  Sequence: QYTTSYDPELTESSGSASHIDCRMSPWSEWSQCDPCLRQMFRSRSIEVFGQFNGKRCTDAVGDRRQCVPTEPCEDAEDDCGNDFQCSTGRCIKMRLRCNGDNDCGDFSDEDDCESEPRPPCRDRVVEESELARTAGYGINILGMDPLSTPFDNEFYNGLCNRDRDGNTLTYYRRPWNVASLIYETKGEKNFRTEHYEEQIEAFKSIIQEKTSNFNAAISLKFTPTETNKAEQCCEETASSISLHGKGSFRFSYSKNETYQLFLSYSSKKEKMFLHVKGEIHLGRFVMRNRDVVLTTTFVDDIKALPTTYEKGEYFAFLETYGTHYSSSGSLGGLYELIYVLDKASMKRKGVELKDIKRCLGYHLDVSLAFSEISVGAEFNKDDCVKRGEGRAVNITSENLIDDVVSLIRGGTRKYAFELKEKLLRGTVIDVTDFVNWASSINDAPVLISQKLSPIYNLVPVKMKNAHLKKQNLERAIEDYINEFSVRKCHTCQNGGTVILMDGKCLCACPFKFEGIACEISKQKISEGLPALEFPNEK

• Full Name: complement component 9
• Calculated Molecular Weight: 63kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: NM_001737.4
• Gene Symbol: C9
• Gene ID (NCBI): 735
• RRID: AB_3743814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P02748
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The final consequence of complement activation is the formation of the membrane attack complex (MAC), a multiprotein assembly that perforates cell membranes, forming transmembrane channels. The MAC plays a critical role in complement-mediated hemolysis and inflammasome activation (PMID: 27444648). Complement component 9 (C9) is 1 of 5 late-acting proteins of complement that participate in the assembly and function of the MAC. C9 may be cleaved by thrombin to generate fragments C9a (25 kDa) and C9b (37 kDa) (PMID: 7523406).