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C9orf117 Polyclonal antibody, PBS Only (21012-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 21012-1-AP (same clone as 21012-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21012-1-AP (C9orf117 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 21012-1-AP (same clone as 21012-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21012-1-AP (C9orf117 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 21012-1-AP (same clone as 21012-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21012-1-AP (C9orf117 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.
IF Staining of A549 using 21012-1-AP (same clone as 21012-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C9orf117 antibody (21012-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

C9orf117 Polyclonal antibody, PBS Only

Name: C9orf117 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 21012-1-PBS

C9orf117 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 21012-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AFAF, Cilia- and flagella-associated protein 157, EQTN, Equatorin

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

21012-1-PBS targets C9orf117 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15234 Product name: Recombinant human C9orf117 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC141809 Sequence: MAPKKSVSKAGKELEVKKKGGKKEPVVAVEPPLAKEMKEFYHIQIRDLEDRLARYQRKWDELAVQEKMFRQEFEQLANNKKEIVAFLKRTLNQQVDEITDLNEQLQNLQLAKEMEKDAFEAQLAQVRHEFQETKDQLTTENIILGGKLAALEEFRLQKEEVTDKFTLLEEQVRKQENEFRDYAYNLEKKSVLDKDRLRKEIIQRVNLVANEFHKVTTNRMWETTKRAIKENNGITLQMARVSQQGMKLLQENEQLKGRQNNLCKQLELLENTQKVMARHKRGHQKIILMLTKKCQEQQQDTKEAEELRLLLSQLEQRSLQLQVDNQALKSQRDQLSLQLEQQQVDLQRLQ Predict reactive species
Full Name	chromosome 9 open reading frame 117
Calculated Molecular Weight	520 aa, 61 kDa
Observed Molecular Weight	60 kDa
GenBank Accession Number	BC141809
Gene Symbol	C9orf117
Gene ID (NCBI)	286207
RRID	AB_3669353
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5JU67
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

C9orf117 (Cilia- and flagella-associated protein 157, CFAP157) protein localizes to basal bodies and interacts with tubulin and the centrosomal protein CEP350 (PMID: 27965440).