C9orf156 Polyclonal antibody

C9orf156 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20475-1-AP

Synonyms

C9orf156, HSPC219, NAP1, Nef associated protein 1, Thioesterase NAP1

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Validation Data Gallery

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  WB analysis of HeLa using 20475-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20475-1-AP (C9orf156 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20475-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20475-1-AP (C9orf156 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20475-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20475-1-AP (C9orf156 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 20475-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20475-1-AP (C9orf156 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 20475-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20475-1-AP (C9orf156 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20475-1-AP is C9orf156 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C9orf156 fusion protein Ag14335
• Full Name: chromosome 9 open reading frame 156
• Calculated Molecular Weight: 441 aa, 49 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: BC002863
• Gene Symbol: C9orf156
• Gene ID (NCBI): 51531
• RRID: AB_10665553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.