C9orf7 / CACFD1 Polyclonal antibody

C9orf7 / CACFD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21330-1-AP

Synonyms

C9orf7, CACFD1, Calcium channel flower homolog, D9S2135, FWE, hFWE, PSEC0248, UNQ3071/PRO9903

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Validation Data Gallery

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  WB analysis using 21330-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21330-1-AP (C9orf7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21330-1-AP targets C9orf7 / CACFD1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C9orf7 / CACFD1 fusion protein Ag15336
• Full Name: chromosome 9 open reading frame 7
• Calculated Molecular Weight: 172 aa, 18 kDa
• Observed Molecular Weight: ~25 kDa
• GenBank Accession Number: BC030558
• Gene Symbol: C9orf7
• Gene ID (NCBI): 11094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calcium Channel Flower Domain Containing 1 (CACFD1) also named as C9orf7 or human Flower protein (hFWE). In Drosophila, cell selection uses a cell selection mechanism based on 'fitness fingerprints', which allow it to delay ageing, prevent developmental malformations and replace old tissues during regeneration. At the molecular level, these fitness fingerprints consist of combinations of Flower membrane proteins. Proteins that indicate reduced fitness are called Flower-Lose, because they are expressed in cells marked to be eliminated. It has different isoforms, the calculated MW rang from 14-25 kDa. 21330-1-AP can detect a band around 25 kDa.(PMID:31341286)