CA12 Recombinant antibody

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  WB analysis using 85595-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85595-3-RR (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85595-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85595-3-RR against Human CA12 were performed. The affinity constant is below 1 pM.

CA12 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85595-3-RR

Clone No.243053B2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CA XII, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII

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Tested Applications

• Positive WB detected in: A549 cells, mouse kidney tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85595-3-RR targets CA12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CA12 fusion protein Ag7107
• Full Name: carbonic anhydrase XII
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC000278
• Gene Symbol: CA12
• Gene ID (NCBI): 771
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43570
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA12 belongs to the alpha-carbonic anhydrase family. It reverses the hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.