CA12 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 85595-3-RR (same clone as 85595-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85595-3-RR (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85595-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01993-1

  Sandwich ELISA standard curve of MP01993-1, Human CA12 Recombinant Matched Antibody Pair - PBS only. 85595-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7107. 85595-2-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

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  Sample test of MP01993-1

  The mean CA12 concentration was determined to be 93.99 ng/mL in A549 cell extract based on a 3.10 mg/mL extract load.

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  Cytometric bead array standard curve of MP01993-1

  Cytometric bead array standard curve of MP01993-1, CA12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85595-3-PBS. Detection antibody: 85595-2-PBS. Standard: Ag7107. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85595-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85595-3-RR against Human CA12 were performed. The affinity constant is below 1 pM.

CA12 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85595-3-PBS

Clone No.243053B2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CA XII, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII

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Product Information

85595-3-PBS targets CA12 as part of a matched antibody pair:

MP01993-1: 85595-3-PBS capture and 85595-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CA12 fusion protein Ag7107
• Full Name: carbonic anhydrase XII
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC000278
• Gene Symbol: CA12
• Gene ID (NCBI): 771
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43570
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CA12 belongs to the alpha-carbonic anhydrase family. It reverses the hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.