CALCA/Calcitonin Monoclonal antibody

• 

  WB analysis of TT using 68774-1-Ig

  TT cells were subjected to SDS PAGE followed by western blot with 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of TT using 68774-1-Ig

  WB result of CALCA/Calcitonin antibody (68774-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-CALCA/Calcitonin transfected TT cells.

• 

  IHC staining of human thyroid cancer using 68774-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 68774-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• KD/KO Validated

CALCA/Calcitonin Monoclonal Antibody for WB, IHC, ELISA

Cat No. 68774-1-Ig

Clone No.1A12A1

Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

CALCA/CGRP, CALCA, Calcitonin, 1A12A1, CALC1

---

Tested Applications

• Positive WB detected in: TT cells
• Positive IHC detected in: human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

68774-1-Ig targets CALCA/Calcitonin in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26141

  Product name: Recombinant human CALCA protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 26-116 aa of BC093753

  Sequence: APFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSSLDSPRSKRCGNLSTCMLGTYTQDFNKFHTFPQTAIGVGAP

• Full Name: Calcitonin
• Calculated Molecular Weight: 141 aa, 15 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC093753
• Gene Symbol: CALCA
• Gene ID (NCBI): 796
• RRID: AB_3670424
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P01258
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calcitonin is a peptide hormone consisting of 32 amino acids, primarily synthesized by the parafollicular cells (C cells) of the human thyroid gland. It plays a crucial role in regulating calcium levels in the blood by decreasing them. Calcitonin opposes the actions of parathyroid hormone, which increases blood calcium levels. Calcitonin works through a G protein-coupled receptor, known as the calcitonin receptor, which predominantly transmits signals via the cAMP and PLC/IP3 pathways. Its primary physiological effects are on osteoclasts and the tubular epithelium of kidneys.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IF	Nat Commun; Brown adipose tissue secretes OLFM4 to coordinate sensory and sympathetic innervation via Schwann cells; Authors - Mingqiang Lai
mouse	WB,IF	J Headache Pain; Migraine-induced cochlear injury triggers ZBP1-mediated PANoptosis via CGRP signaling.; Authors - Wandi Xu