CALCA/Calcitonin Monoclonal antibody, PBS Only (Capture)

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  WB analysis of TT using 68774-1-Ig (same clone as 68774-1-PBS)

  TT cells were subjected to SDS PAGE followed by western blot with 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68774-1-PBS in a different storage buffer formulation.

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  Sample test of MP50293-1

  Cytometric bead array in cell lysate using MP50293-1, CALCA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68774-1-PBS. Detection antibody: 68774-2-PBS. Cell lysate: TT, HepG2.

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  IHC staining of human thyroid cancer using 68774-1-Ig (same clone as 68774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68774-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 68774-1-Ig (same clone as 68774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68774-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50293-1

  Cytometric bead array standard curve of MP50293-1, CALCA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68774-1-PBS. Detection antibody: 68774-2-PBS. Standard:Ag26141. Range: 0.781-100 ng/mL.

CALCA/Calcitonin Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Cat No. 68774-1-PBS

Clone No.1A12A1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

CALCA/CGRP, CALCA, Calcitonin, 1A12A1, CALC1

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Product Information

68774-1-PBS targets CALCA/Calcitonin as part of a matched antibody pair:

MP50293-1: 68774-1-PBS capture and 68774-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26141

  Product name: Recombinant human CALCA protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 26-116 aa of BC093753

  Sequence: APFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSSLDSPRSKRCGNLSTCMLGTYTQDFNKFHTFPQTAIGVGAP

• Full Name: Calcitonin
• Calculated Molecular Weight: 141 aa, 15 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC093753
• Gene Symbol: CALCA
• Gene ID (NCBI): 796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P01258
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Calcitonin is a peptide hormone consisting of 32 amino acids, primarily synthesized by the parafollicular cells (C cells) of the human thyroid gland. It plays a crucial role in regulating calcium levels in the blood by decreasing them. Calcitonin opposes the actions of parathyroid hormone, which increases blood calcium levels. Calcitonin works through a G protein-coupled receptor, known as the calcitonin receptor, which predominantly transmits signals via the cAMP and PLC/IP3 pathways. Its primary physiological effects are on osteoclasts and the tubular epithelium of kidneys.