CALCOCO2 Monoclonal antibody

CALCOCO2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2F2C2

Cat no : 66401-1-Ig

Synonyms

CALCOCO2, NDP52, Nuclear domain 10 protein 52, Nuclear dot protein 52



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66401-1-Ig targets CALCOCO2 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CALCOCO2 fusion protein Ag2866
Full Name calcium binding and coiled-coil domain 2
Calculated molecular weight 446 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC015893
Gene symbol CALCOCO2
Gene ID (NCBI) 10241
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CALCOCO2, also named as NDP52, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat Calcoco2 has some isoforms with MW 28-40 kDa and 67 kDa. Human CALCOCO2 has some isoforms with MW 43-47 kDa and 52-55 kDa,

Protocols

Product Specific Protocols
WB protocol for CALCOCO2 antibody 66401-1-IgDownload protocol
IHC protocol for CALCOCO2 antibody 66401-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.

Authors - Norihiko Furuya