NDP52 Monoclonal antibody

NDP52 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC

Conjugate

Unconjugated

CloneNo.

2F2C2

Cat no : 66401-1-Ig

Synonyms

CALCOCO2, NDP52, Nuclear domain 10 protein 52, Nuclear dot protein 52



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, human skeletal muscle tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66401-1-Ig targets NDP52 in WB, IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NDP52 fusion protein Ag2866
Full Name calcium binding and coiled-coil domain 2
Calculated molecular weight 446 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC015893
Gene symbol CALCOCO2
Gene ID (NCBI) 10241
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,

Protocols

Product Specific Protocols
WB protocol for NDP52 antibody 66401-1-IgDownload protocol
IHC protocol for NDP52 antibody 66401-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.

Authors - Norihiko Furuya