- Featured Product
- KD/KO Validated
NDP52 Monoclonal antibody
NDP52 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2F2C2
Cat no : 66401-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, Jurkat cells, human skeletal muscle tissue |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
Product Information
66401-1-Ig targets NDP52 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | NDP52 fusion protein Ag2866 |
Full Name | calcium binding and coiled-coil domain 2 |
Calculated molecular weight | 446 aa, 52 kDa |
Observed molecular weight | 52 kDa |
GenBank accession number | BC015893 |
Gene symbol | CALCOCO2 |
Gene ID (NCBI) | 10241 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,
Protocols
Product Specific Protocols | |
---|---|
WB protocol for NDP52 antibody 66401-1-Ig | Download protocol |
IHC protocol for NDP52 antibody 66401-1-Ig | Download protocol |
IF protocol for NDP52 antibody 66401-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
mBio The nonstructural protein 1 of respiratory syncytial virus hijacks host mitophagy as a novel mitophagy receptor to evade the type I IFN response in HEp-2 cells |